Prof. Dr. Marcel Ameloot  

Current Position

2000 - ... full professor at the Hasselt University

Hasselt University
Campus Diepenbeek
Agoralaan - Building D
B-3590 Diepenbeek
Belgium
Phone: + 32 11 268 546
Fax: + 32 11 268 299
E-mail: marcel.ameloot@uhasselt.be

Former Positions

1975-1984: teaching assistant at the UHasselt
1984 (March-December): postdoctoral stay in U.S.A. (Prof. L. Brand, The Johns Hopkins University, Department of Biology, Baltimore). 
1984-1991: staff member at the UHasselt
1991-1999: assistant professor at the UHasselt

Education

Master (physics): 1975 (KULeuven)
Certificat de biophysique 1980 (Université de Liège)
PhD: 1984 (UHasselt - Campus Diepenbeek)

Teaching

Experimental Methods en techniques in physics 
Physical models in medical sciences 
Methods and techniques in cell physiology and cell biology 
Optics and microscopy 

Publications

Recent articles in peer review journals (2000 - ...)

Molski A, Boens N, Ameloot M. Identifiability of complex photophysical models with transients. Journal of Molecular Liquids.86 (2000) 45-51.

Boens N., Szubiakowski J.P., Novikov E. Ameloot M. Testing the identifiability of a model for reversible intermolecular two-state excited-state processes. Journal of . Chemical Physics. 112 (2000) 8260 - 8266

Despa S., Steels P., Ameloot M. Fluorescence lifetime microscopy of the sodium indicator sodium-binding benzofuran isophthalate in HeLa cells. Analytical Biochemistry 280 (2000) 227 - 241

Despa S., Vecer J., Steels P., Ameloot M. Lifetime-based fluorescence microscopy of the ion indicator Sodium Green in HeLa cells. Analytical Biochemistry 281 (2000) 159 – 175

F. Jans, F. Vandenabeele, M. Helbert, I. Lambrichts, M. Ameloot, P. Steels A simple method for obtaining functionally and morphologically intact primary cultures of the medullary thick ascending limb of Henle’s loop (MTAL) from the rabbit kidneys. Pflugers Archiv. European Journal of Physiology, 440 (2000) 643 - 651

M. Buntinx, M. Ameloot, P. Steels, P. Janssen, R. Medaer, P. Geusens, J. Raus, P. Stinissen Interferon-g-Induced Calcium Influx in T lymphocytes of Multiple Sclerosis and Rheumatoid Arthritis Patients: A Complementary Mechanism for T Cell Activation? Journal of Neuroimmunology, 124 (2002) 70 – 82

Smets I, Ameloot M, Steels P, Van Driessche W. Loss of cell volume regulation during metabolic inhibition in renal epithelial cells (A6): role of intracellular pH. Am J Physiol Cell Physiol. 2002 Aug;283(2):C535-44. 

M. Buntinx, P. Stinissen, P. Steels, M. Ameloot, J. Raus Immune-mediated oligodendrocyte injury in multiple sclerosis: molecular mechanisms and therapeutic interventions. Critical Reviews in Immunology, 22 (2002) 391-424

M. Buntinx, J. Vanderlocht, N. Hellings, F. Vandenabeele, I. Lambrichts, J. Raus, M. Ameloot, P. Stinissen, P. Steels Characterization of three human oligodendroglial cell lines as a model to study oligodendrocyte injury: morphology and oligodendrocyte-specific gene expression Journal of Neurocytology 32 (2003) 25-38

Wenmackers S, Haenen K, Nesladek M, Wagner P, Michiels L, vandeVen M, Ameloot M. Covalent immobilization of DNA on CVD diamond films Physica Status Solidi A – Applied Research 199 (2003) 44-48

Smets I, Caplanusi A, Despa S, Molnar Z, Radu M, vandeVen M, Ameloot M, Steels P Ca²⁺ uptake in mitochondria occurs via the reverse action of the Na⁺/Ca²⁺ exchanger in metabolically inhibited MDCK cells American Journal of Physiology – Renal Physiology 286 (2004) F784-F794 

Former Publications (1982 - 1999)

Ameloot M., Hendrickx H. Criteria for model evaluation in the case of deconvolution calculations. Journal of Chemical Physics (1982) 76: 4419-4432.

Ameloot M., Hendrickx H. Extension of the performance of Laplace convolution in the analysis of fluorescence decay curves. Biophysical Journal (1983) 44: 27-38.

van der Meer W., Pottel H., Herreman W., Ameloot M., Hendrickx H., Schröder H. Effect of the orientational order on the decay of the fluorescence anisotropy in membrane suspensions . A new approximate solution of the rotational diffusion equation. Biophysical Journal (1984) 46: 515-523.

Ameloot M., Hendrickx H., Herreman W., Pottel H., Van Cauwelaert F., van der Meer W. Effect of the orientational order on the decay of the fluorescence anisotropy in membrane suspensions. Experimental verification on unilamellar vesicles and lipid/a-lactalbumin complexes. Biophysical Journal (1984) 46: 525-539.

Beechem J.M., Ameloot M., Brand L. Global analysis of fluorescence decay surfaces: excited state reactions. Chemical Physics Letters (1985) 120: 466-472.

Beechem J.M., Ameloot M., Brand L. Global and target analysis of complex decay phenomena. Analytical Instrumentation (1985) 14: 379-402.

Ameloot M., Beechem J.M., Brand L. Simultaneous analysis of multiple fluorescence decay curves by Laplace transforms. Deconvolution with reference and excitation profiles. Biophysical Chemistry (1986) 23: 155-171.

Pottel H., Herreman W., van der Meer W., Ameloot M. On the significance of the fourth rank orientational order parameter of fluorophores in membranes. Chemical Physics (1986) 102: 37-44.

Ameloot M., Beechem J.M., Brand L. Compartmental modeling of excited-state reactions: identifiability of the rate constants from fluorescence decay surfaces. Chemical Physics Letters (1986) 129: 211-219. 

van Langen H., Levine Y.K., Ameloot M., Pottel H. Ambiguities in the interpretation of time-resolved fluorescence anisotropy measurements on lipid vesicle systems. Chemical Physics Letters (1987) 140: 394-400.

Boens N., Ameloot M., Yamazaki I., De Schryver F.C. On the use and the performance of the delta function convolution method for the estimation of fluorescence decay parameters. Chemical Physics (1988) 121: 73-86.

Boens N., De Schryver F.C., Ameloot M. Laser pulse fluorometry as a tool in polymer research Makromolekulare Chemie. Macromolecular Symposia (1988) 18: 93-100.

Janssens L.D., Boens N., Ameloot M., De Schryver F.C. A systematic study of the global analysis of multi-exponential fluorescence decay surfaces using reference deconvolution. Journal of Physical Chemistry (1990) 94: 3564-3576.

Ameloot M., Boens N., Andriessen R., Van den Bergh V., De Schryver F.C. Non a priori analysis of fluorescence decay surfaces of excited-state processes. 1. Theory. Journal of Physical Chemistry (1991) 95: 2041-2047.

Andriessen R., Boens N., Ameloot M., De Schryver F.C. Non a priori analysis of fluorescence decay surfaces of excited-state processes. 2. Intermolecular excimer formation of pyrene. Journal of Physical Chemistry (1991) 95: 2047-2058.

Khalil M.M.H., Boens N., Van der Auweraer M., Ameloot M., Andriessen R., Hofkens J., De Schryver F.C. Compartmental analysis of the fluorescence decay surface of the exciplex formation between 1-methylpyrene and triethylamine. Journal of Physical Chemistry (1991) 95: 9375-9380.

Andriessen R., Ameloot M., Boens N., De Schryver F.C. Non a priori analysis of fluorescence decay surfaces of excited-state processes. 3. Intermolecular excimer formation of pyrene quenched by iodomethane. Journal of Physical Chemistry (1992) 96: 314-326.

Boens N., Andriessen R., Ameloot M., Van Dommelen L., De Schryver F.C. Kinetics and identifiability of intramolecular two-state excited-state processes. Global compartmental analysis of the fluorescence decay surface. Journal of Physical Chemistry (1992) 96: 6331-6342. 

Van den Bergh V., Boens N., De Schryver F.C., Ameloot M., Gallay J., Kowalczyk A. One-step parameter estimation of the acid-base equilibria in the ground and excited-state of 2-naphthol by global compartmental analysis of the fluorescence decay curves. Chemical Physics (1992) 166: 249-258. 

Boens N., Ameloot M., Hermans B., De Schryver F.C., Andriessen R. Kinetics and identifiability of intramolecular two-state excited-state processes with added quencher. Global compartmental analysis of the fluorescence decay surface. Journal of Physical Chemistry (1993) 97: 799-808.

Matjko J., Jenei A., Matyus L., Ameloot M., Damjanovich S. Mapping of cell surface protein-patterns by combined fluorescence anisotropy and energy transfer. Journal of Photochemistry and Photobiology B (1993) 19: 69-73.

Crutzen M., Ameloot M., Boens N., Martin R. Negri, De Schryver F. Global analysis of unmatched polarized fluorescence decay curves. Journal of Physical Chemistry (1993) 97: 8133-8145.

Boens N., Van Dommelen L., Ameloot M. Specifying upper and lower bounds on the rate constants of intramolecular two-state excited-state processes. Global compartmental analysis of the fluorescence decay surface. Biophysical Chemistry (1993) 48: 301-313.

Van Dommelen L., Boens N., Ameloot M., De Schryver F.C., Kowalczyk A Species-associated spectra and upper and lower bounds on the rate constants of reversible intramolecular two-state excited-state processes with added quencher. Global Compartmental analysis of the fluorescence decay surface. Journal of Physical Chemistry (1993) 97: 11738-11753.

Ameloot M., Van den Bergh V., Boens N., De Schryver F.C., Steels P. A new calibration equation for ratiometric fluorescent ion indicators: application to FURA-2. Journal of Fluorescence (1993) 3: 169-171.

Wittouck N., Negri R.M., Ameloot M., De Schryver F.C. AOT reversed micelles investigated by fluorescence anisotropy of cresyl violet. Journal of Physical Chemistry (1994) 116: 10601-10612.

Hermans B., De Schryver F.C., Boens N., Ameloot M., Jérome , Teyssié Ph., Goethals E., Schacht E. Global tricompartmental analysis of the fluorescence decay surface of the charged fluorescent probe N,N,N-trimethyl-3-(1-pyrenyl)-1-propanaminium perchlorate. Journal of Physical Chemistry (1994) 98: 13583-13593.

De Schryver F.C., Boens N., Van der Auweraer M., Viaene L., Reekmans S., Hermans B., Van Stam J., Gehlen M., Berghmans H., Ameloot M. Excited-state probing of associative and covalent macromolecules. Pure and Applied Chemistry (1995) 67: 157-165.

van den Bergh V., Boens N., De Schryver F.C., Ameloot M., Steels P., Gallay J., Vincent M., Kowalczyk A. Photophysics of the fluorescent Ca2+ indicator Fura-2 Biophysical Journal (1995) 68: 1110-1119. 

De Backer S., Negri M.R., De Feyter S., Dutt G.B., Ameloot M., De Schryver F.C., Müllen K., Holtrup F. Fluorescence depolarisation of 2,5,8,11-tetra-t-buytlperylene globally analysed upon excitation in S1 and Sn. Chemical Physics Letters (1995) 233: 538-544.

Vereb G., Dale R.E., Matyus L., Bene L., Panyi G., Bacsó Z., Balazs M., Matkó J., Pieri C., Ameloot M., Szöllosi J., Gaspar R., Damjanovich S. Plasma-membrane-bound macromolecules are dynamically aggregated to form nonrandom codistribution patterns of selected functional elements. Do pattern recognition processes govern antigen presentation and intercellular interactions? Appendix: resonance energy transfer. Journal of Molecular Recognition (1995) 8: 237-246.

Van Dommelen L., Boens N., Ameloot M., De Schryver F.C. Distinction between different competing kinetic models of irreversible intramolecular two-state excited-state processes with added quencher. Global compartmental analysis of the fluorescence decay surface. Journal of Physical Chemistry (1995) 99: 8959-8971.

De Backer S., Dutt G.B., Ameloot M., De Schryver F.C., Müllen K., Holtrup F. Fluorescence anisotropy of 2,5,8,11-tetra-tertiary-butyl-perylene and 2,5,10,13-tetra-tertiary-butyl-terrylene in alkanes and alcohols. Journal of Physical Chemistry (1996) 100: 512-518.

Biasutti M.A., De Feyter S., De Backer S., Dutt G.B., De Schryver F.C., Ameloot M., Schlichting P., Müllen K. Steady-state and time-resolved fluorescence anisotropy of 2,9-di-n-pentyl-5,6,11,12-tetraazo-5,6,11,12-tetrahydrocoronene -5,6,11,12-tetracarboxy-bis-n-heptadecyl-imide. Chemical Physics Letters (1996) 248: 13-19.

Panyi G., Gaspar R., Krasznai Z., ter Horst J.J., Ameloot M., Aszalos A., Steels P., Damjanovich S. Immunosuppressors inhibit voltage-gated potassium channels in human peripheral blood lymphocytes. Biochemical Biophysical Research Communications (1996) 221: 254 - 258.

Dutt G.B., Ameloot M., Bernik D., Negri R.M., De Schryver F.C. Global analysis of unmatched polarized fluorescence decay curves of systems exhibiting a residual anisotropy at long times. Journal of Chemical Physics (1996) 100: 9751 - 9761.

Kowalczyk A., Boens N., Meuwis K., Ameloot M. Potential misevaluation of the ground-state dissociation constant from fluorimetric titrations: application to the ion indicators SBFI, PBFI and Fura-2. Analytical Biochemistry (1997) 245: 28 - 37.

Molski A., Boens N., Ameloot M. Transient effects can change identifiability criteria for complex excited-state processes. Chemical Physics Letters (1997) 266: 139 - 144.

Dutt G.B, Boens N., Kowalczyk A., De Schryver F.C., Ameloot M. Experimental design in global compartmental analysis of reversible intramolecular two-state excited-state processes with added quencher. Journal of Physical Chemistry (1997) 101: 1993 - 2002.

Bene L., Szöllosi J., Balazs M., Matyus L., Gaspar R., Ameloot M., Dale R.E., Damjanovich S. Major histocompatibility complex class I protein conformation altered by transmembrane potential changes. Cytometry (1997) 27: 353 - 357.

Molski A., Boens N., Ameloot M. Transient effects and the identifiability of excited-state processes. Journal of Physical Chemistry A (1998) 102: 807 - 811.

Meuwis K., Boens N., De Schryver F.C., Ameloot M., Gallay J., Vincent M. Photophysics of SBFO, a fluorescent indicator for Na+. Journal Physical Chemistry B (1998) 102: 641 - 648.

Van Dommelen L. , Boens N., Ameloot M. Time-resolved fluorescence of glucagon studied by global compartmental analysis. Journal of Physical Chemistry B (1998) 102: 3287 - 3294.

Zeiske W., Smets I., Ameloot M., Steels P., Van Driessche W. Intracellular pH-shifts in cultured kidney (A6) cells: effects on apical Na+ transport. Am. J. Physiology. (Cell Physiol. 46) (1999) 277: C469-C479. 

Reports in books

Ameloot M., De Smedt H., Hendrickx H. Fluorescent anisotropy experiments on hog renal brush border membrane vesicles. in "Fluorescent Biomolecules: Methodologies and Applications" (1989) pp.430-432. Eds. D.M. Jameson, G.D. Reinhart, Plenum Press. 

Beechem J.M., Gratton E., Ameloot M., Knutson J.R., Brand L. The global analysis of fluorescence intensity and anisotropy decay data: second generation theory and programs. in Topics in Fluorescence Spectroscopy Principles (1991) Volume 2: pp.241-305. Ed. J.R. Lakowicz, Plenum Press, New York.

ter Horst J.J., Ameloot M., Gallay J., Vincent M., De Schrijver F.C., Steels P. Determination of the rate constant of the fluorescent probe C. SNAFL-1 in the excited state. In "Optical Biopsy and Fluorescence Spectroscopy and Imaging" (1995) 2324: pp. 329-340. Eds. R. Cibeddu, R. Marchesini S.R. Mordon, K. Svanberg, H.H. Rinneberg, G. Wagnieres Proceedings of S.P.I.E.

Plasek J., ter Horst J.J., Ameloot M., Steels P. The effect of protein binding on the calibration curve of the pH indicator BCECF. In "Fluorescence microscopy and fluorescent probes."(1996): pp. 120-125. Plenum, New York. 

Plásek J., ter Horst J.J., Ameloot M., Steels P. Confocal fluorimetry with a standard epifluorescence microscope. in "Fluorescence microscopy and fluorescent probes Vol. 2," [Proceedings of the conference Fluorescence Microscopy & Fluorescent Probes, Prague, 9 - 12 April 1997], ed. J. Slavik, Plenum, New York, 1998, p. 75 - 80. 

Reports in Proceedings

Ameloot M., Hendrickx H. Statistical methods in deconvolution calculations. Proceedings of the meeting "Deconvolution and Reconvolution" (1982): pp.463-477. Ed. M. Bouchy, E.N.S.I.C., Nancy, France. 

Ameloot M., Hendrickx H. A non-iterative Laplace deconvolution method with the power to combine various experiments. Proceedings of the meeting "Deconvolution and Reconvolution" (1982): pp. 227-297. Ed. M. Bouchy, E.N.S.I.C., Nancy, France. 

Ameloot M., Boens N. Investigation of ground state interaction through fluorescence decay surfaces of excited-state reactions. in "Time-Resolved Laser Spectroscopy in Biochemistry II" (1990) Vol 1204: pp.506-513. Ed. J.R. Lakowicz, Proceedings of S.P.I.E., S.P.I.E. Bellingham. 

Boens N., Janssens L.D., Ameloot M., De Schryver F.C. Model testing capacity of global analysis of single photon timing data. in "Time-Resolved Laser Spectroscopy in Biochemistry II" (1990) Vol 1204: pp.456-467. Ed. J.R. Lakowicz, Proceedings of S.P.I.E., S.P.I.E. Bellingham. 

Boens N., Ameloot M., Andriessen R., Van Dommelen L., De Schryver F.C., Hermans B. Compartmental analysis of the fluorescence decay surface of intramolecular two-state excited-state processes with added quencher. in "Time-Resolved Laser Spectroscopy in Biochemistry III" (1992) Vol 1640: pp.342-353. Ed. J.R. Lakowicz, Proceedings of S.P.I.E., S.P.I.E. Bellingham. 

Ameloot M., Van den Bergh V., Boens N., De Schryver F.C., Steels P. A new calibration equation for ratiometric fluorescent ion indicators: application to FURA-2.
Journal of fluorescence (1993) 3: 169 - 171.

De Schryver F.C., van Stam J., Gehlen M.H., Van der Auweraer M., Boens N., Reekmans S., Negri R.M., Wittouck N., Bernik D., Ameloot M., Faes H., Noukakis D. Microchemistry.Luminescence spectroscopy and microdomains. Proceedings of the JRDC-KUL Joint International Symposium on Spectroscopy and Chemistry in Small Domains (1994): pp. 415-430. Eds. Masuhara H. et al., Elsevier, Amsterdam. 

Boens N., Van Dommelen L., De Schryver F.C., Ameloot M. Evidence for reversible excited-state process in tryptophan zwitterion. in "Time-Resolved Laser Spectroscopy in Biochemistry IV" (1994) Vol 2137: pp.400-411. Ed. J.R. Lakowicz, Proceedings of S.P.I.E., S.P.I.E. Bellingham.

Van den Bergh V., Meuwis K., Boens N., De Schryver F.C., Vincent M., Gallay J., 
Ameloot M. Photophysical study of the Ca2+ indicator Fura-2 and the K+ indicator PBFI. in "Time-Resolved Laser Spectroscopy in Biochemistry IV" (1994) Vol 2137: pp.400-411. Ed. J.R. Lakowicz, Proceedings of S.P.I.E., S.P.I.E. Bellingham. 

ter Horst J.J., Ameloot M., Gallay J., Vincent M., De Schryver F.C., Steels P. Determination of the rate constants of the fluorescent probe C.SNAFL-1 in the excited state. in "Optical Biopsy and Fluorescence Spectroscopy and Imaging" (1995) Vol. 2324: pp.329-340. Eds. R. Cibeddu, R. Marchesini, S.R.Mordon, K. Svanberg, H.H. Rinneberg, G. Wagnieres, Proceedings of S.P.I.E., S.P.I.E. Bellingham. 

Kowalczyk A., Boens N., Ameloot M. Methods in Enzymology Vol. 278, eds. L. Brand and M. L. Johnson, Academic Press, 1997, p. 94-113. 

Reports in special series

Ameloot M., Boens N., Andriessen R., Van den Bergh V., De Schryver F.C. Compartmental analysis of fluorescence decay surfaces of excited-state processes. Methods in Enzymology (1992) Vol. 210: pp. 314-340. Eds. L. Brand and M. L. Johnson, Academic Press.

Ameloot M. Laplace deconvolution of fluorescence decay surfaces. Methods in Enzymology (1992) Vol. 210: pp.279-304. Eds. L. Brand and M. L. Johnson, Academic Press.

Kowalczyk A., Boens N., Ameloot M. Determination of the ground-state dissociation constant by fluorescence spectroscopy. Methods in Enzymology (in press)